Release Date: 01 December, 2002
Paperback
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From Book News, Inc.
Written for both graduate students and experienced investigators, this manual describes the general principles, analytical methods, and protocols involved in the study of proteomics. Simpson (Joint Proteomics Laboratory, Australia) begins with polyacrylamide gel electrophoresis for the separation of proteins in complex mixtures and the preparation of cellular extracts. Further protocols are provided for reversed-phase liquid chromatography, amino- and carboxyl-terminal sequence analysis, and peptide mapping. The largest chapter consists of 13 protocols for using mass spectrometry to identify proteins. Each protocol lists the reagents and equipment required and step-by-step instructions for performing the method.Copyright © 2004 Book News, Inc., Portland, OR
Book Description
Completion of sequencing of the human genome, as well as those of many other organisms, has now opened the door for exploration of the proteome, the many thousands of interacting proteins in a given organism. Critical to tackling the complexity of the proteome is a workable strategy using reliable and tested protocols for identifying, isolating, and quantifying proteins in cells and cell pathways and for performing functional assays. Proteins and Proteomics: A Laboratory Manual, authored by Richard Simpson of the Ludwig Institute for Cancer Research, Melbourne, provides the first authoritative and wide–ranging protocol-based approach to proteomics. Presented as a logical strategy for analyzing proteomes, Proteins and Proteomics provides information about protein structure and numerous methods for the preparation and analysis of protein samples ranging from electrophoresis and mass spectrometry to protein chips and informatics. Extensive background information and references are provided regarding the theoretic aspects of the techniques presented as well as their applications. This manual is an essential laboratory tool for genetics and molecular biology investigators moving from studies of genomics and genotype to those of proteomics and phenotype. It is invaluable for cell biologists, developmental biologists, and neuroscientists exploring the intricacies of the proteome. It will also be an excellent resource for protein chemists and biochemists.
Book Info
Ludwig Institute, Melbourne, Australia. Text is aimed at research isolating proteins and peptides for subsequent proteomic analysis. Covers the proteomics, methods, and technologies currently used in the laboratory. Provides background information for each accompanying protocol. Two-tone format. Softcover, hardcover listed in approval plan week 2003-04. Rating 4.0
An OK book but reinvents the wheelTo be fair this is a good and useful book, but at a higher price than the already excellent published (2002) laboratory proteomics by Westermeier and Naven, save your dollars. The latter goes for half the price and serves the same purpose with excellent illustrations and protocols.The "must have" book for the practicing proteomics lab .This is a outstanding book, covering all aspects of sample preparation and experimental techniques involved in protein and peptide analysis by gels, liquid chromatography and mass spectrometry. Each chapter gives detailed protocols complete with tips, reagent lists, short tutorials (printed in blue colored information panels) and references. The protocols will work for you just as they are written and will give you a place to start in customizing and adapting them to your own circumstances (and you certainly will do that!). As with any fast moving field an updated second edition will be welcome around 2006, but by then, I'll be happy to replace my dog-eared copy for a fresh one (or maybe not, I don't really think a book as good as this will ever be obsolete). I recommend you buy the paperback edition, it will serve you well and save you some $$$. |
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