From Book News, Inc.
After explaining the basic science behind the Polymerase Chain Reaction, this lab manual provides protocols for sample preparation, RT-PCR methods, detection of PCR products, analysis of differential expression, cloning, and mutagenesis. Appendices offer information on licensing, modification of oligonucleotides, hazards, and suppliers. The 31 chapters represent the work of scientists from around the country.Copyright © 2004 Book News, Inc., Portland, OR
Immunology Today
"There is little doubt that this manual should find its place on the shelves of many laboratories." --This text refers to the Paperback edition.
Trends in Biochemical Sciences
"The generally high standard of writing and editing has made reading the entire manual refreshingly easy." --This text refers to the Paperback edition.
Book Description
From its first-published account in 1985, the polymerase chain reaction has become a standard research tool in a wide range of laboratories. Its impact has been felt in basic molecular biological research, clinical research, forensics, evolutionary studies, and the Human Genome Project. The PCR technique originally conceived by Nobel laureate Kary Mullis has proven to be exceptionally adaptable and has been transformed into a myriad array of methods, each with different applications. PCR Primer: A Laboratory Manual introduces the complex world of PCR by beginning at an accessible level and then moving to more advanced levels of application. First, the practical requirements for performing PCR and other amplification techniques in the lab are introduced and then the basic aspects of the technique are explained by exploring important issues such as sample preparation, primer design, efficiency, detection of products, and quantitation. Protocols for a wide range of PCR and amplification techniques—each written by an expert investigator—are presented for cloning, sequencing, mutagenesis, footprinting, library construction and screening, exon trapping, differential display, and expression, and these include RT-PCR, RNA PCR, LCR, multiplex PCR, panhandle PCR, capture PCR, expression PCR, 3' and 5' RACE, immune PCR, in situ PCR, and ligation-mediated PCR. Each protocol is augmented by analysis and troubleshooting sections and complete references.--This text refers to the Paperback edition.
Book Info
National Institute of Allergy and Infectious Diseases, Bethesda, MD. Summarizes the advances in polymerase chain reaction as well as the more well-established methods. Introduces the basics for PCR and sample preparation, provides protocols for RT-PCR, and analyzes differential expression and cloning. Previous edition: c1995. Softcover, hardcover available. Rating 5.0
Best PCR manual there is!I have used two different protocols from this book, 1, RACE-PCR and 2, mutagenesis.These two methods were described very well, both theoretically and practically. The protocols were described in detail, were easy to follow, and they work! I'm sure that the other protocols in this book also work equally well. If you do PCR, you need this book! |
